P4HB anticorps

N° du produit ABIN6658053

N° du produit (Fournisseur): 100-401-g29

Cet anticorps Lapin Polyclonal détecte spécifiquement P4HB dans WB, IHC, IP et IF. Il présente une réactivité envers Rat.

Aperçu rapide pour P4HB anticorps (ABIN6658053)

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp P4HB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Détail du produit anti-P4HB anticorps

N° du produit (Fournisseur): 100-401-g29

Fournisseur: Rockland

Fonction: PDI Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with PDI from Human, Mouse, Rat, Dog, Hamster, Monkey, Guinea pig, Bovine, Sheep, Pig, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-PDI Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Stérilité: Sterile filtered

Immunogène: PDI Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against rat PDI synthetic peptide conjugated to KLH.

Isotype: IgG

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:4000

Commentaires:

Anti-PDI Antibody is tested for use in WB, IP, IF microscopy and IHC. Expect a band approximately ~58kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur P4HB

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Autre désignation: PDI

Sujet:

Synonyms: PDA2, PDI, PDIA2, PDIP, pancreatic protein disulfide isomerase, Protein disulfide-isomerase, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta

Background: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins. Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins. PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase. PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol. PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus. It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

Gene Name: P4hb

ID gène: 287164

NCBI Accession: NP_001099245

UniProt: P04785

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism